A Study on Relationships between Various Quantitative Characteristics for Using Availability Quantitative Trait Loci Mapping

Özge Şahin, Orhan Kavuncu


In this study, Tribolium confusum Jacquelin du Val, 1868 (Coleoptera: Tenebrionidae) lines were selected as live material and they were used for quantitative trait locus analysis. The two of T. confusum lines were selected randomly and they were used for breeding process. During the experiments the insects were held in the mediums consisting of 95% flour and 5% yeast mixture which were placed in the incubators adjusted to suitable moisture and 32.50C temperature. Pupal weight for the selected marker loci was examined whether there are effective on quantitative traits. The examination of the differences between pupae weight of generations were analyzed by ANOVA in Minitab. After this analyze a significant difference in the pupal weight between these lines was not found. According to the results it was concluded that the material used in this study was inconvenient to detect a quantitative trait locus and a mapping study. Still, in terms of examining for the implementations of methods further hybridizations and analysis were carried out.


Flour Beetle; QTL; Pupa Weight

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Brown SJ, Denell RE & Beeman RW (2003). Beetling around the genome. Genetics Research Cam-bridge 82 (3): 155-161.

Düzgüneş O & Ekingen HR (1983). Genetics. Faculty of Agriculture Publications of Ankara Universi-ty, Ankara, 343 pp.

Falconer DS & Mackay TFC (1996). Introduction to Quantitative Genetics (Fourth Edition). Addi-son Wesley Longman Limited, Harlow, Essex, 464 pp.

Hall HG (1986). DNA differences found between Afri-canized and European honeybees. Proceedings of the National Academy of Sciences of the United States of America 83 (13): 4874-4877.

Hall HG (1990). Parental analysis of intogressive hy-bridization between African and European hon-eybees using nuclear DNA RFLPs. Genetics 125: 611-621.

Lander E & Kruglyak L (1995). Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nature Genetics 11: 241-247.

Semeao AA, Campbell JF, Beeman RW, Whitworth RJ, Sloderbeck PE & Lorenzen MD (2010). Genetic structure of Tribolium castaneum populations in mills. Julius-Kühn-Archiv 425: 85-89.

Sokoloff A (1977). The Biology of Tribolium: with Special Emphasis on Genetic Aspects. Claren-don Press, Oxford, 628 pp.

Şahin Ö (2016). Tribolium confusum’da çeşitli özel-likler arasındaki ilişkilerin QTL haritalamada kullanılabilirliği üzerine bir araştırma. Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Ankara, Türkiye.

Tanksley SD (1993). Mapping polygenes. Annual Re-view of Genetics 27: 205-233.

Tribolium Genome Sequencing Consortium (2008). The genome of the model beetle and pest Tribo-lium castaneum. Nature 452 (7190): 949-955.

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA & Tingey SF (1990). DNA polymorphism ampli-fied by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18 (22): 6531-6535.

Xu S (2002). QTL Analysis in Plants. In Camp NJ & Cox A (Eds) Quantitative Trait Loci: Methods and Protocols. Humana Press, Totowa, New Jersey, 376 pp.

Zhong D, Pai A & Yan G (2003). Quantitative trait loci for susceptibility to tapeworm infection in the red flour beetle. Genetics 165 (3): 1307-1315.

DOI: https://doi.org/10.15316/SJAFS.2019.154


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