Effects of Bacteria and IBA on the Rooting of Bitter Orange (Citrus aurantium L.) and Trifoliate Orange (Poncirus trifoliata Raf.) Cuttings

Mustafa Çınar, Lütfi Pırlak, Güçer Kafa, Metin Turan


In this study, the effects of Agrobacterium rubi A-18 and Bacillus OSU-142 bacterial strains alone and in combination with 1000, 2000, 4000 ppm IBA on rooting of bitter orange and trifoliate orange citrus rootstock in softwood, semi-hardwood and hardwood cuttings were investigated. In the case of IBA solution, Agrobacterium rubi A-18 and Bacillus OSU-142 were prepared in solution at a concentration of 1x109 bacteria / ml and applied to steel. Application is made in the mist propagation system, in the perlite are planted in the environment. Steels kept in the fogging environment for 3 months were removed at the end of this period and rooting rates (%), callus formation rates (%) and survival rates (%) were determined. According to the results of the research, the rooting rates of the steels taken at different periods were found to be low. The highest rooting rates of bitter orange softwood cuttings were 2000 ppm IBA + OSU-142 (6.67%), semi-hardwood cuttings OSU-142 (20.00%), and 4000 ppm IBA + OSU-142 (20.00%) in hardwood cuttings. It has been not rooting in trifoliate orange softwood cuttings, the highest rooting was in the case of semi-hardwood cuttings with 4000 ppm IBA + OSU-142 (26.67%), in hardwood cuttings 1000 ppm IBA + OSU-142, 2000 ppm IBA + OSU-142, 4000 ppm IBA + OSU-142, 1000 ppm IBA + A-18, 2000 ppm IBA + A-18 and 4000 ppm IBA + A-18 (20.00%). According to the cuttings pick-up period, the rooting rates of bitter orange are not different between the periods, in the trifoliate orange the hardwood cuttings were found to be higher. As a result, it can be said that plant growth promoting bacteria and IBA applications have no effect on rooting in the softwood, semi-hardwood and hardwood cuttings of the bitter orange and trifoliate orange citrus rootstocks, it can be said that studies on rooting in these rootstocks can be useful in different bacterial breeds


Bitter orange Trifoliate orange IBA Plant growth promoting bacteria Rooting of cutting

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Ahmad, T., Ur-Rahman, H., Ahmed, C.H. and Laghari M.H., 2005, Effect of culture media and growth regulators on micropropagation of peach rootstock GF 677, Pakistan Journal of Bo-tany, 35(3), 331-338.

Antonopoulou, C., Dimassi, K., Therios, I., Chatzissav-vidis, C. and Tsirakoglou, V., 2005, Inhibitory ef-fect of riboflavin (Vitamin B2) on the in vitro roo-ting and nutrient concentration of explants of pe-ach rootstock GF 677 (Prunus amygdalus x P. per-sica), Scientia Horticulturae, 106, 268-272.

Arıkan, Ş., İpek, M., Eşitken, A., Pırlak, L., 2015, Ef-fects of IBA and Plant Growth Promoting Rhizobacteria (PGPR) on Rooting and Root Growth of Myrtle (Myrtus communis L.) Stem Cuttings. En-vironmentally Friendly Agriculture and Forestry for Future Generations, XXXVI CIOSTA & CIGR Sec-tion V Conference, pp: 42-45.

Bassil, N. V., Proebsting, W. M., Moore, L. W. and Lig-htfoot, D. A. J. H., 1991, Propagation of hazelnut stem cuttings using Agrobacterium rhizogenes, HortScience, 26 (8), 1058-1060.

Bhusal, R.C., Mizutani, F., Rutto, K.L., 2003, Effects of juvenility on the rooting of trifoliate orange (Poncirus trifoliata Raf.) stem cuttings. J. Jpn. Soc. Hort. Sci. 72, 42-45.

Cooper, W. C., 1935, Hormones in relation to root formation on stem cuttings. Plant Physiol., 10, 789-794.

De Klerk, G-J., Ter Brugge, J, Marinova, S., 1997, Effec-tiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’, Plant Cell. Tiss. Org. 49:39-44.

Ercişli, S., Eşitken, A., Cangi, R., Şahin, F., 2003, Ad-ventitious root formation of kiwifruit in relation to sampling date, IBA and Agrobacterium rubi inocu-lation. Plant Growth Regulation 41:133-137.

Ercişli, S., Eşitken, A., Şahin, F., 2004, Exogenous IBA and inoculation with Agrobacterium rubi stimulate adventitious root formation on hardwood stem cut-tings of two rose genotypes. HortScience 39(3):533-534.

Erturk, Y., Ercisli, S., Haznedar, A., Cakmakci, R. 2011. Effects of plant growth promoting rhizobac-teria (PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings. Biol Res 43: 91-98.

FAO, 2019, www.fao.org (Access date, 01.02.2019).

Gülcan, R., 1991, Meyve ağaçlarında anaç ıslahı, Türkiye 1. Fidancılık Sempozyumu, 185-193.

Habermann, G., Alvarez, R.D.F., Modesto, J.C. Fortes, A.M.D., Rodrigues, J.D., Ono, E.O., 2006. Rooting of healthy and CVC affected ‘Valencia’ sweet orange stem cuttings through the use of plant regu-lators. Braz. Arch. Biol. Technol. 49, 29-36.

Hartmann, H.T., Kester, D.E. and Davies, F.T. Jr., 1990, Plant Propagation, Principles and practices. Prentice-Hall, Inc. New Jersey, 647 p.

Hatta, M., Beyl, C.A., Garton, S. and Diner, A.M., 1996, Induction of roots on jujube softwood cut-tings using Agrobacterium rhizogenus. Journal of Hort. Sci., 71(6):881-886.

Jacob, M. and Handam, I., 1992, Use of benefical bacteria for Pelargonium zonale. Gartenbaumaga-zin. 1(3):105-107.

Kınık, E. and Çelikel, F. G. 2017, Bakteri ve oksin uygulamalarının kuşburnu bitkisinin çelikle çoğal-tılması üzerine etkileri, Türk Tarım Gıda Bilim ve Teknolojisi Dergisi, 5 (13), 1714-1719.

Larraburu, E. E., Carletti, S. M., Rodríguez Cáceres, E.A., Llorente, B.E., 2007, Micropropagation of photinia employing rhizobacteria to promote root development, Plant Cell Reports, 26 (6), 711-7.

Mendilcioğlu, K., 1999, Subtropik İklim Meyveleri (Turunçgiller), Ege Üniv. Ziraat Fak. Yay. Ders Not-ları: 9/5.

Nagarajan, P., Ratha, N.V., Kandasamye, D., Oblisami, G. and Jayaraj, S., 1989, Effect of combined inocu-lation of Azospirillum brasilense and Glomus fas-ciculatum on mullbery. Madras Agricultural Jour-nal. 76(11):601-605.

Pirlak, L. and Baykal, Y., 2009. Effect of IBA and Bacteria (Agrobacterium rubi and Bacillus OSU 142) on the Rooting of M9 Apple Rootstock Cut-tings. 1st International Syposium on Sustainable Development, pp. 129-134, Sarajevo.

Rom, R.C., 1987, Roots, Rootstocks for Fruit Cropps, Rom, C.R. and Carlson, R.F. (eds). A Wiley-Intersience Publ., U.S.A., pp 107-144.

Rugini, E. and Fedelli, E., 1990, Olive (Olea europaea L.) as an oilseed crop. Ed. J.P.S. Bajaj, Biotechno-logy in Agriculture and Forestry, Vol. 10: Legumes and oilseed crops. I.Springer- verlag Berlin, Heider-berg.

Sarmast, M. K., Salehi, H., Khosh-Khui, M., 2012, In vitro rooting of Araucaria excelsa R. BR. var. Glauca using Agrobacterium rhizogenes, Journal of Central European Agriculture, 13(1), 123-130.

Singh, Z., Sandhu, A.S., Dhillon, B.S., 1988, Rooting and sprouting of stem cuttings of Citrus jambhiri in response to IBA and cyclophosphamide. Acta Hort. 227, 145-149.

Teixeira, D. A., Alfenas A. C., Mafia, R. G., Ferreira, E. M., De Siqueira L., Maffia, L. A. and Mounteer A. H., 2007, Rhizobacterial promotion of eucalypt ro-oting and growth, Brazilian Journal of Microbio-logy, 38:118-123.

TUİK, 2019, www.tuik.gov.tr (Access date, 01.05.2019),

Uzun, A., Seday, Ü., 2011, Farklı IBA dozlarının farklı turunçgil anaçlarının odun çeliklerinin köklenmeleri üzerine etkileri. Erciyes Üniv. Fen Bil. Ens. Der. 212-216.

Webster, A.D. and Looney, N.E., 1996, Cherries. Crop Physiology, Production and Uses. CAB Internatio-nal, Wallingford 513 pp.

DOI: http://dx.doi.org/10.15316/SJAFS.2019.164


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