Detection of Some Virus Diseases of Edible Seed Squash (Cucurbita pepo L.) in Nevşehir Province, Turkey

Serkan Yeşil

Abstract


Squash is a member of Cucurbitaceae family. It is grown for fresh consuming and its seeds are used as a snack in Turkey like some Mediterranean countries and Germany, Hungary, Austria and China. Cucurbita pepo L. is mostly used for cultivating squash seeds in Turkey. Also, a small amount of seeds obtain from Cucurbita moschata Duch (Butternut squash or pumpkin). Virus diseases are one of the most destructive diseases on squash which is grown for seeds in Nevşehir province. In this study, it was aimed to determine the virus infections in major squash growing areas in Nevşehir province. Totally 134 plant samples with common virus symptoms like mosaic, curling, blistering, mottling, distortion, shoestring, stunting and vine decline were collected from squash plants during 2018. In this study. Double-Antibody Sandwich Enzyme-Linked Immunosorbent Assay(DAS-ELISA) method is used for identifying the virus infections on the plant samples. According to the results of the DAS-ELISA, 97.76 % of plant samples were infected with Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus-2 (WMV), Cucurbit aphid borne yellow virus (CABYV), Cucumber mosaic cucumovirus (CMV), Papaya ringspot potyvirus-watermelon strain (PRSV-W) and Squash mosaic comovirus (SqMV). WMV was predominant in the research area with the ratio of 89.55 %. ZYMV was the second important virus disease in the surveyed area and it was detected on the samples at the ratio of 57.46 %. Also, mixed infections of those virus infections were detected commonly in squash. Especially, ZYMV+WMV (40.29 %) and WMV+ZYMV+PRSV-W (8.20 %) mixed infections were common.

Keywords


DAS-ELISA; Edible seed squash; Nevşehir; WMV-2; Virus

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References


Abou-Jawdah Y, Sobh H, El-Zammar S, Fayyad A, Lecoq H (2000). Incidence and management of vi-rus diseases of cucurbits in Lebanon. Crop Protec-tion, 19:217-224.

Al-Ali EA, Al-Hashash H, Al-Aqeel H, Ben HA (2013). Multiple important plant viruses are present on vegetable crops in Kuwait. Journal of Clinical Trials 3, 136. doi:10.4172/2167-0870.1000136.

Anonymous (2019). 2016-2018 yılları Nevşehir ili çerezlik kabak bitkileri üretim değerleri. Nevşehir İl, Gıda, Tarım ve Hayvancılık Müdürlüğü

Caciagli P (2010). Vegetable Viruses. Pages 479-487 in: Desk Encyclopedia of Plant and Fungal Virology. Mahy, BWJ and Van Regenmortel, MHV eds. Academic Press, Linacre House, Jordan Hill, Ox-ford, OX2 8DP, UK.

Campbell RN (1971). Squash mosaic virus. Descrip-tions of Plant Viruses. [http://www.dpvweb.net/dpv/showdpv.php?dpvno=43] (Date of access: 20.03.2019).

Clark MF, Adams AN (1977). Characteristics of the microplate method of enzyme-linked immuno-sorbent assay for the detection of plant viruses. Journal of General Virology 34:475-783.

Çağlar BK, Guldur ME, Yılmaz MA (2004). First report of Squash mosaic virus in Turkey. Journal of Plant Pathology 86 (2), 177-180.

D’Arcy CJ, Domier LL (2005). Luteoviridae, p891-900. In Fauquet CM, Mayo MA, Maniloff J, Des-selberger U, Ball LA (ed). Virus taxonomy, VIIIth report of the ICTV. Elsevier/Academic Press, Lon-don, United Kingdom.

Davis RF, Yılmaz MA (1984). First Report of Zucchini Yellow Mosaic Virus on Watermelon and Squash in Turkey. Plant Disease 68:537

Davis RI, Thomas JE, McMichael LA, Dietzgen RG, Callaghan B, James AP, Gunua TG, Rahamma S (2002). Plant virus surveys on the island of New Guinea and adjacent regions of northern Australia. Australasian Plant Pathology 31: 385-390.

Dogimont C, Slama S, Martin J, Lecoq H, Pitrat M (1996). Sources of resistance to Cucurbit aphid-borne yellows Luteovirus in a melon germ plasm collection. Plant Disease 80:1379-1382. Doi:10.1094/PD-80-1379.

Erdiller G, Ertunç F (1988). Identification of muskmel-on viruses in Ankara province. Journal of Turkish Phytopathology 17:47-56.

Fidan U (1995). Virus diseases of vegetables in green-houses in Izmir and Mugla. Journal of Turkish Phy-topathology 24:7-14.

Francki RIB, Mossop DW, Hatta T (1979). Cucumber mosaic virus. Descriptions of Plant Viruses. [http://www.dpvweb.net/dpv/showdpv.php?dpvno=213]. (Date of access: 20.03.2019).

Gray S, Cilia M, Ghanim M (2014). Circulative, “nonpropagative” virus transmission: an orchestra of virus-, insect-, and plant-derived instruments. Advances in Virus Research 89:141-199. doi:10.1016/B978-0-12-800172-1.00004-5.

Gümüş M, Erkan S, Yorgancı U, Duman I (2001). The investigation on the determination of viruses in the seeds of certain vegetables. Proceedings of the IX. Phytopathology Congress of Turkey. 3-8 September 2001, Tekirdağ, Turkey. 190-197.

Kaper JM, Waterworth HE (1981). Cucumoviruses. In: Handbook of Plant virus Infections and Compara-tive Diagnosis. E. Kurstak, ed. Pages 257-332. Elsevier/North Holland Biomedical Press, Amster-dam, the Netherlands.

Kızmaz MZ, Sagır A, Baloglu S (2016). Diyarbakır ve Mardin İlleri Kabakgil Üretim alanlarında Görülen Viral Hastalıkların Yaygınlıklarının ve Etmen-lerinin Belirlenmesi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 53 (4):397-406.

King A, Adams M, Carstens E, Lefkowitz E (2012). Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Academic Press, London, United Kingdom

Korkmaz F, Topkaya Ş, Yanar Y (2018). Tokat Kaba-kgil Üretim Alanlarında Enfeksiyon Oluşturan Virüslerin Belirlenmesi. Gaziosmanpaşa Bilimsel Araştırma Dergisi 7 (2): 46-56.

Köklü G, Yılmaz O (2006). Occurrence of cucurbit viruses on field-grown melon and watermelon in the Thrace region of Turkey. Phytoprotection, 87:123-130.

Kurcman S (1977). Determination of virus diseases on cultural plants in Turkey. Journal of Turkish Phyto-pathology 6:25-46.

Lecoq H, Pitrat M, Clement M (1981). Identification et caractérisation d’un potyvirus provoquant la mala-die du rabougrissement jaune du melon. Agronomie 1: 827-834.

Lecoq H, Bourdin D, Wipf-Scheibel C, Bon M, Lot H, Lemaire O, Herrbach E (1992). A new yellowing disease of cucurbits caused by a Luteovirus, Cucur-bit aphid-borne yellows virus. Plant Pathology 41: 749-761. doi: 10.1111/j.1365-3059.1992.tb02559.x.

Lecoq H (1999). Epidemiology of Cucurbit aphid-borne yellows virus, p 243-248. In Smith HG, Baker H (ed). The Luteoviridae. CABI Publishing, Wallingford, United Kingdom.

Lisa V, Lecoq H (1984). Zucchini yellow mosaic virus. Descriptions of Plant viruses. [http://www.dpvweb .net/dpv/ showdpv.php?dpvno=282]. (Date of ac-cess: 20.03.2019).

Luis-Arteaga M, Alvarez JM, Alonso-Prados JL, Bernal JJ, Garcia-Arenal F, Lavina A, Batlle A, Moriones E (1998). Occurrence, distribution, and relative incidence of mosaic viruses infecting field-grown melon in Spain. Plant Disease 82: 979-982.

Makkouk KM, Lesemann DE (1980). A severe mosaic of cucumbers in Lebanon caused by Watermelon mosaic virus-1. Plant Disease 64: 799-801.

Malandraki I, Vassilakos N, Xanthis C, Kontosfiris G, Katisand NI, Varveri C (2014). First report of Mo-roccan watermelon mosaic virus in Zucchini crops. Plant Disease 98(5): 702.

Massumi H, Samei A, Pour AH, Shaabanian M, Rahimian H (2007). Occurrence, Distrubution, and Relative Incidence of Seven Viruses Infecting Greenhouse-Grown Cucurbits in Iran. Plant Disease 91:159-163.

Michelle H, Veronique B (2018). Targeted disruption of aphid transmission: a vision for the management of crop diseases caused by Luteoviridae members. Current Opinion in Virology 33:24-32. doi: 10.1016/j.coviro.2018.07.007.

Nameth ST, Dodds JA, Paulus AO, Laemmlen FF (1986). Cucurbit viruses of California: an ever-changing problem. Plant Disease 70: 8-11.

Nogay A, Yorgancı U (1984). Investigations on the identification, seed transmission and host range of viruses infecting the culture plants in the Cucurbi-taceae in Marmara region. 1. The identification of viruses infecting cucurbits in Marmara region. Journal of Turkish Phytopathology 13:9-28

Özaslan M, Aytekin T, Bas B, Kilic H, Afacan ID, Dag DS (2006) Virus Diseases of Cucurbits in Gazian-tep, Turkey. The Plant Pathology Journal 5, 24-27.

Provvidenti R (1996). Diseases caused by viruses. In: Zitter TA, Hopkins DL, Thomas CE (eds), Com-pendium of Cucurbit Diseases, Minnesota, USA, APS Press, pp. 37-45.

Purcifull D, Edwardson J, Hiebert E, Gonsalves D (1984a). Papaya ringspot virus. Descriptions of Plant Viruses. [http://www.dpvweb.net/dpv/showdpv.php?dpvno =292]. (Date of access: 20.03.2019).

Purcifull D, Hiebert E, Edwardson J (1984b). Water-melon mosaic virus 2. Descriptions of Plant Viruses. [http://www.dpvweb.net/dpv/showdpv.php?dpvno=293]. (Date of access: 20.03.2019).

Sammons B, Barnett OW, Davis RF, Mizuki MK (1989). A survey of viruses infecting yellow sum-mer squash in South Carolina. Plant Disease 73: 401-404.

Şevik MA, Balkaya A, (2015). Samsun, Sinop ve Bolu İllerindeki Bal Kabağı (Cucurbita moschata Duch) Popülasyonlarına Ait Tohum Örneklerinde Virüslerin Tanılanması ve Bulunma Durumlarının Belirlenmesi. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi 32(3):70-77.

Topkaya S, Ertunc F (2012). Current status of virus infections in cucurbit plantations in Ankara and Antalya provinces. Proceedings of the Xth EUCAR-PIA Meeting on Genetics and Breeding of Cucurbi-taceae, 759-762.

Topkaya, S, Desbiez, C, Ertunc, F (2019). Presence of cucurbit viruses in Ankara and Antalya province and molecular characterization of coat protein gene of zucchini yellow mosaic virus turkish isolates. Fresenius Environmental Bulletin 28(4): 2442-2449.

TÜİK (2019). 2018 yılı Türkiye çerezlik kabak ekim alanı ve üretim miktarları. www.tuik.gov.tr (Date of access: 14.07.2019)

Yeşil S, Ertunç F (2012). Virus Diseases of Cucurbits in Konya Province. In: Proceedings of the Xth EU-CARPIA meeting on genetics and breeding of Cu-curbitaceae (eds. Sari, Solmaz and Aras), October, 15-18th, 2012, Antalya, Turkey, pp. 791-796.

Yeşil S (2014). Virus Diseases of Edible Seed Squash (Cucurbita pepo L.) in Konya Province. In: Book of Proceedings Fifth International Scientific Agricultural Symposium (AGROSYM-2014), October, 23-26th, 2014, Jahorina, Sarajevo, Bosnia-Herzegovina, 226 p. ISBN: 978-99955-751-9-9

Yeşil S (2019a). Some Virus Diseases of Edible Seed Squash (Cucurbita pepo L.) in Aksaray Province, Turkey. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 29:63-71.

Yeşil S (2019b). Detection of Viruses on Edible Seed Squash (Cucurbita pepo L.) in Yozgat Province, Turkey. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 9 (3):1202-1219.

Yeşil S, Ertunç F (2015). Reservoir Weeds of Cucurbit Viruses in Central Anatolia. In: Proceedings of the International Congress on Applied Biological Sci-ences (ICABS-2015). September 16-20, 2015, Skopje, Macedonia, 36 p.

Yeşil S, Ertunç F (2016). Seed Transmission of Some Cucurbit Viruses in Turkey. In: Proceedings of the International Conference on Agricultural, Biologi-cal and Environmental Sciences (ICABES-2016). December 14-16, 2016, Pattaya, Thailand, 104 p. ISBN:978-93-84468-80-4

Yılmaz MA, Özaslan M, Baloğlu S (1991). Çukurova bölgesinde yetistiriciligi yapılan kavun, karpuz ve hıyar bitkilerine zararlı yeni bir virüs hastalığı. Turkiye VI. Fitopatoloji Kongresi. Izmir-Turkiye, 387-391.

Yılmaz MA, Lecoq H, Abak K, Baloglu S, Sarı N (1992). Türkiye’de kabakgil sebze türlerinde zarar yapan virüsler. Türkiye I. Ulusal Bahçe Bitkileri Kongresi, İzmir, Bildiriler kitabı, 439-442.

Yuki VA, Rezende JAM, Kitajima EW, Barroso PAV, Kuniyuki H, Groppo GA, Pavan MA (2000). Oc-currence, distribution, and relative incidence of five viruses infecting cucurbits in the state of Sao Paulo, Brazil. Plant Disease 84: 516-520.

Zitter AT (2002). A checklist of major weeds and crops as natural hosts for plant viruses in the Northeast. Department of Plant Pathology, Cornell University, Ithaca, NY, New York. (Date of access: 20.03.2019) http://vegetablemdonline.ppath.cornell.edu/Tables/ WeedHostTable.html#Keys.




DOI: http://dx.doi.org/10.15316/SJAFS.2020.194

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