Doubled Haploid Production in Cereals Using Microspore Culture

Neslihan Doruk, Nejdet Kandemir, Sabri Gökmen

Abstract


Doubled haploids are extremely useful in plant breeding since they provide rapid homozygosity. However, the success rate of doubled haploid production in cereals is still not high enough, and there is a special problem involving the formation of high percentage of albino plants. Nevertheless, the success rate in microspore culture in cereals is higher than classical anther cultures, and the method has the advantage of spontaneous chromosome doubling. On the other hand, this method has some critical stages such as pretreatments and microspore isolation, and these stages need to be optimized for the successful use of the technique in plant breeding. For this aim, there have been studies in recent years about combining the pretreatment practices, supplementing growth media with a variety of ingredients, improving the various co-culturing practices and decreasing the albino plant percentage. This technique has been commonly used in the world especially in barley and wheat breeding. Improving the success rate of the technique will be useful for its integration into modern breeding techniques such as apomictic crops and transgenics.

Keywords


Albino plant Co-cultivation Embryo induction Green plant Microspore isolation Pretreatment

Full Text:

PDF

References


Asif M, Eudes M, Randhawa H, Amundsen E and Spaner D (2014). Induction medium osmolality im-proves microspore embryogenesis in wheat and trit-icale. In Vitro Cellular and Developmental Biology - Plant 50:121–126.

Bashaw EC (1980). Apomixis and its application in crop improvement. In: Fehr WR and Hadley HH (ed) Hybridization of Crop Plants, American Soci-ety of Agronomy Crop Science Society of America Soil Science Society of America, Madison pp. 45–63.

Broughton S (2008). Ovary co-culture improves em-bryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.). Plant Cell Tissue and Organ Culture 95:185–195.

Caredda S, Doncoeur C, Devaux P, Sangwan RS, Clement C (2000). Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.). Sexual Plant Reproduction 13 (2): 95-104.

Chen J, Cui L, Malik AA, Mbira K (2011). In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tissue and Organ Culture 104: 311–319.

Davies PA (2003). Barley isolated microspore culture (IMC) method. In: Maluszynski M, Kasha KJ, For-ster BP, Szarejko I (eds.) Doubled haploid produc-tion in crop plants: A manual, Kluwer, Dordrecht, pp 49–52.

Davies P and Morton S (1998). A comparison of barley isolated microspore and anther culture and the in-fluence of cell culture density. Plant Cell Reports 17: 206–210.

Devaux P and Kasha KJ (2009). Overview of barley doubled haploid production. In: Touraev A, Forster BP and Jain SM (eds.) Advances in Haploid Pro-duction in Higher Plants, Heidelberg, Springer: 47–63.

Esteves P and Belzile FJ (2019). Isolated microspore culture in barley. In: Harwood W. (ed.) Barley: Methods in Molecular Biology, vol 1900. Humana Press, New York, NY.

Esteves P and Belzile F (2014a). Improving the effi-ciency of isolated microspore culture in six-row spring barley: I-optimization of key physical fac-tors. Plant Cell Reports 33 (6): 993–1001.

Esteves P, Clermont I, Marchand S, Belzile F (2014b). Improving the efficiency of isolated microspore culture in six-row spring barley: II-exploring novel growth regulators to maximize embryogenesis and reduce albinism. Plant Cell Reports 33 (6): 871–879.

Ferrie AMR and Caswell KL (2011). Isolated micro-spore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue and Organ Culture 104: 301–309.

Ferrie A M R, Irmen KI, Beattie AD, Rossnagel BG (2014). Isolated microspore culture of oat (Avena sativa L.) for the production of doubled haploids: effect of pre-culture and post-culture conditions. Plant Cell Tissue and Organ Culture 116: 89–96.

Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007). The resurgence of haploids in higher plants. Trends in Plant Science 12: 368–375.

Foroughi-Wehr, B, Mix G, Gaul H and Wilson H M (1976). Plant production from cultured anthers of Hordeum vulgare. L. Z. Pflanzenzucht 77: 198-204.

Gaillard A, Vergne, P, Beckert M (1991). Optimization of maize microspore isolation and culture condi-tions for reliable plant regeneration. Plant Cell Re-ports 10: 55 58.

Germana M A (2011). Anther culture for haploid and doubled haploid production. Trends Plant Science 104: 283–300.

Gland-Zwerger A, Javornik B, Bohanec B, Kreft I (1994). Production of doubled haploid lines through anther culture in barley and triticale. In: Javornik B and Bohanec B (eds.) Proceed. International Collo-quium. Impact of Plant Biotechnology on Agricul-ture. December 5-7, Rogla, Slovenia: 9-14.

Hoekstra S, van Zijderveld M H, Louwerse J D, Heidekamp F, van der Mark F (1992). Anther and microspore culture of Hordeum vulgare L. cv Igri. Plant Science 86: 89-96.

Houben A, Sanei M, Pickering R (2011). Barley dou-bled-haploid production by uniparental chromo-some elimination. Plant Cell Tissue and Organ Culture 104: 321–327.

Hu TC, Ziauddin A, Simion E, Kasha KJ (1995). Iso-lated microspore culture of wheat (Triticum aes-tivum L.) in a defined media. I. Effects of pretreat-ment, isolation methods, and hormones. In Vitro Cell and Developmental Biology 31: 79–83

Humphreys DG and Knox RE (2015). Doubled haploid breeding in cereals. In: Al-Khayri J, Jain S and Johnson D. (eds.) Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools, Springer, Cham.

Islam SSM, Ara I, Tuteja N, Subramaniam, S (2013). Efficient microspore isolation methods for high yield embryoids and regeneration in rice (Oryza sa-tiva L.). International Journal of Biological Science and Engineering Vol: 7 No: 12.

Jacquard C, Wojnarowiez G, Clement C (2003). Anther culture in barley. In: Doubled haploid production in crop plants, Springer, Berlin, Heidelberg, Tokyo, pp. 21-28.

Jacquard C, Asakaviciute R, Hamalian AM, Sangwan R S, Devaux P, Clement C (2006). Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism. Plant Cell Reports 25: 375–381.

Jahne A, Becker D, Brettschneider R, Lörz H. (1994). Regeneration of transgenic, microspore-derived, fertile barley. Theoretical and Applied Genetics 89 (4): 525-33.

Jansky S (2006). Overcoming hybridization barriers in potato. Plant Breeding 125 (1): 1-12.

Kasha KJ, Simion E, Miner M, Letarte J, Hu TC (2003). Haploid wheat isolated microspore culture protocol. In: Maluszynski M, Kasha KJ, Forster BP and Szarejko I (eds.) Doubled haploid production in crop plants: A manual, Kluwer, Dordrecht, pp 77–81.

Köhler F and Wenzel G (1985). Regeneration of isolat-ed barley microspores in conditioned media and tri-als to characterise the responsible factor. Journal of Plant Physiology 121: 181–191.

Letarte J, Simion E, Miner M, Kasha J (2006). Arabi-nogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) mi-crospore culture. Plant Cell Reports 24: 691.

Li H and Devaux P (2001). Enhancement of micro-spore culture efficiency of recalcitrant barley geno-types. Plant Cell Reports 20: 475–481.

Lippmann R, Friedel S, Mock HP, Kumlehn J (2015). The low molecular weight fraction of compounds released from immature wheat pistils supports bar-ley pollen embryogenesis. Frontiers in Plant Sci-ence 6: 498.

Liu W, Zheng MY, Polle EA, Konzak CF (2002). Highly efficient doubled-haploid production in wheat Triticum aestivum L. via induced microspore embryogenesis. Crop Science 42: 686–692.

Makowska K, Oleszczuk S, Zimny J (2017). The effect of copper on plant regeneration in barley micro-spore culture. Czech Journal of Genetics and Plant Breeding 53 (1): 17–22.

Malayeri B E, Noori M, Jafari M (2012). Using the pollen viability and morphology for fluoride pollu-tion biomonitoring. Biological Trace Element Re-search 147: 315–319.

Oleszczuk S, Sowa S, Zimny J (2006). Androgenic response to preculture stress in microspore cultures of barley. Protoplasma 228: 95–100.

Olsen FL (1991). Isolation and cultivation of embryo-genic microspores from barley (Hordeum vulgare L.). Hereditas 115: 255-266.

Ouyang J W, Zhou SM, Jia SE (1993). The response of anther culture to culture temperature in Triticum aestivum. Theoretical and Applied Genetics 66: 101–109.

Patel M, Darvey NL, Marshall DR, Berry JO (2004). Optimization of culture conditions for improved plant regeneration efficiency from wheat micro-spore culture. Euphytica 140: 197–204.

Pechan PM and Keller WA (1989). Induction of micro-spore embryogenesis in Brassica napus L. by gamma irradiation and ethanol stress. In Vitro Cel-lular and Developmental Biology - Animal 25: 1073–1074.

Raina SK and Irfan ST (1998). High-frequency embry-ogenesis and plantlet regeneration from isolated microspores of indica rice. Plant Cell Reports 17: 957–962.

Rajcan I, Boersma JG, Shaw EJ (2011) Plant genetic techniques: Plant Breeder’s Toolbox. Comprehen-sive Biotechnology (Second Edition), Volume 4. Elsevier.

Sestili S and Ficcadenti N (1996). Irradiated pollen for haploid production. In: Jain, SM, Sopory SK and Veilleux RE (eds.) In vitro haploid production in higher plants. Springer, Netherlands, pp. 263–274.

Shariatpanahi ME and Ahmadi B (2016). Isolated microspore culture and its applications in plant breeding and genetics. In: Anis M and Ahmad N (eds.) Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, Singapore.

Soriano M, Li H, Boutilier K (2013). Microspore em-bryogenesis: establishment of embryo identity and pattern in culture. Plant Reproduction 26: 181–196.

Tek A, Stupar R, Nagaki K (2015). Modification of centromere structure: a promising approach for haploid line production in plant breeding. Turkish Journal of Agriculture and Forestry 39 (4): 557-562.

Torp AM and Andersen SB (2009). Albinism in micro-spore culture. In: Touraev A et al. (eds.) Advances in haploid production in higher plants, Springer, New York.

Vizintin L and Bohanec B (2004). In vitro manipula-tion of cucumber (Cucumis sativus L.) pollen and microspores: Isolation procedures, viability tests, germination, maturation. Acta Biologica Cracoviensia Series Botanica 46: 177–183.

Wang HM, Enns JL, Nelson KL (2019). Improving the efficiency of wheat microspore culture methodolo-gy: evaluation of pretreatments, gradients, and epi-genetic chemicals. Plant Cell Tissue and Organ Culture 139: 589–599.

Wojnarowiez G, Caredda S, Devaux P, Sangwan R, Clement C (2004). Barley anther culture: assess-ment of carbohydrate effects on embryo yield, green plant production and differential plastid de-velopment in relation with albinism. Journal of Plant Physiology 161: 747–755.

Zhao JP, Simmonds DH, Newcomb W (1996). Induc-tion of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas. Planta 198: 433-439.

Zheng MY, Liu W, Weng Y, Polle E, Konzak CF (2001). Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals. Plant Cell Reports 20: 685–690

Zheng M Y (2003). Microspore culture in wheat (Triti-cum aestivum) – doubled haploid production via in-duced embryogenesis. Plant Cell Tissue and Organ Culture 73: 213–230.

Ziauddin A, Simion E, Kasha KJ (1990). Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. Igri. Plant Cell Reports 9: 69-72

Ziegler G, Dressler K, Hess D (1990). Investigations on the anther culturability of four German spring wheat cultivars and the influence of light on regen-eration of green vs. albino plants. Plant Breeding 105: 40-46.

Zieliński K, Krzewska M, Żur I, Juzoń K, Kopeć P, Nowicka A, Moravčiková J, Skrzypek E, Dubas E (2020). The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cul-tures of rye (Secale cereale L.). Plant Cell, Tissue and Organ Culture 140: 577–592.

Zur I, Dubas E, Krzewska M, Zieliński K, Fodor J, Janowiak F (2019). Glutathione provides antioxida-tive defence and promotes microspore-derived em-bryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.). Plant Cell Re-ports 38: 195–209.




DOI: http://dx.doi.org/10.15316/SJAFS.2020.212

Refbacks

  • There are currently no refbacks.


Creative Commons Lisansı
Bu eser Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.