The Identification of Genetic Variation in Insulin-Like Growth Factors-I (IGF-I) Gene Region in Some Turkish Sheep Breeds

Mustafa Tawfeeq, İsmail Keskin, Fatma İlhan


Insulin-like growth factors (IGFs) is known as peptides with important metabolic effects required for cellular growth and metabolism. IGF-I is synthesized in liver tissue under control of growth hormone (GH) and released to blood. In the process of GH to accelerate growth, IGF-I occupies a large place. IGF-I clearly showed its important effects on the growth of animal studies. In this study, the IGF-I genes of sheep were amplified by polymerase chain reaction (PCR) in sheep breeds (Akkaraman, Kivircik, Awassi, Sakiz, Daglıc, Morkaraman, Malya Karayaka (15 to 20 sheep per breed)) reared in Turkey. Informative restriction fragment length polymorphisms (RFLPs) were obtained with HaeII enzyme. The digestion of IGF-I gene with HaeII produced two alleles and three genotypes. Genotype frequencies were 59%, 19%and 22% for AA, BB and AB genotypes, respectively. Alleles frequencies were 0.70 for A allele and 0.30 for B allele. This study indicates the genetic profiles of the IGF-1 gene in native Turkish sheep breeds.


Growth hormone Insulin-like growth factor (IGF) Sheep Polymerase chain reaction (PCR Restriction fragment length polymorphism (RFLP)

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Alakilli SY, Mahrous KF, Salem LM, Ahmed ES (2012). Genetic polymorphism of five genes asso-ciated with growth traits in goat, African Journal of Biotechnology, 11 (82), 14738-14748.

Ali A, Javed K., Akram M, Dawood M, Saleem A. (2016). Polymorphism of insulın-like growth factor-1 gene and its association with growth rate in desi chicken of Pakistan, JAPS, Journal of Animal and Plant Sciences, 26 (3), 858-861.

Behzadi S, Miraei-Ashtiani SR, Sadeghi M, Zamani P, Abdoli R (2015). Association of IGF-I gene poly-morphisms with carcass traits in Iranian Mehraban sheep using SSCP analysis, Iran. J. Appl. Anim. Sci., 5, 121–126.

Ge W, Davis ME, Hines HC, Irvin KM. Simmen RC (2001). Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle, J. Anim. Sci., 79, 1757–1762.

Grochowska E, Borys B, Janiszewski P, Knapik J. Mroczkowski S (2017). Effect of the IGF-I gene polymorphism on growth, body size, carcass and meat quality traits in Coloured Polish Merino sheep. Archives Animal Breeding, 60(2), 161-173.

He J, Zhang B, Chu M, Wang P, Feng T, Cao G, Di R, Fang L, Huang D. Tang Q (2012). Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep, Molecular biology reports, 39 (10), 9801-9807.

Bondy CA, Underwood LE, Clemmons DR, Guler HP, Bach MA, Skarulis M (1994). Clinical uses of insu-lin-like growth factor I, Annals of internal medicine, 120 (7), 593-601.

Daughaday WH (1997). Sulfation factor revisited: The one-two punch of insulin-like growth factor-I action on cartilage. The Journal of laboratory and clinical medicine, 4(129), 398-399.

Kaymakci M, Engindeniz S (2010). Türkiye’de Keçi Yetiştiriciliği: Sorunlar ve Çözümler. Ulusal Keçicilik Kongresi 24-26 Haziran 2010, Bildiriler Kitabı, Çanakkale, 1-25

Kopchick JJ, List EO, Kelder B, Gosney ES. Berryman DE (2014). Evaluation of growth hormone (GH) ac-tion in mice: discovery of GH receptor antagonists and clinical indications, Molecular and cellular en-docrinology, 386 (1-2), 34-45.

Le Roith D, Bondy C, Yakar S, Liu JL. Butler A (2001). The somatomedin hypothesis: Endocrine reviews, 22 (1), 53-74.

Li H F, Zhu WQ, Chen KW, Wu X, Tang QP. Gao YS (2008). Associations between GHR and IGF-1 gene polymorphisms, and reproductive traits in Wen-chang chickens, Turkish Journal of Veterinary and Animal Sciences, 32 (4), 281-285.

Miller SA, Dykes DD, Polesky HF (1988). A simple salting out procedure for extracting DNA from hu-man nucleated cells. Nucleic Acids Research. 16 (3), 1215.

Othman OE, Abdel-Samad MF, El-Maaty NAA. (2016). Evaluation of insulin-like growth factor-I gene polymorphism in Egyptian small ruminant breeds, African Journal of Biotechnology, 15 (48), 2714-2719.

Pereira AP, Alencar MM, Oliveira HN, Regitano LC (2005). Association of GH and IGF-1 polymor-phisms with growth traits in a synthetic beef cattle breed, Genetics and Molecular Biology, 28 (2), 230-236.

Siadkowska E, Zwierzchowski L, Oprzadek J, Strzalkowska N, Bagnicka E, Krzyzewski J (2006). Effect of polymorphism in IGF-1 gene on produc-tion traits in Polish Holstein-Friesian cattle, Anim Sci Pap Rep, 24 (3), 225-237.

Yilmaz A, Davis ME, Hines HC, Chung H (2005). Detection of two nucleotide substitutions and puta-tive promoters in the 5’flanking region of the ovine IGF-I gene, J Appl Genet, 46 (3), 307-309.



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