Determination of Calpastatin Gene Polymorphism in Kivircik Crossbred Ewes by PCR-RFLP Method

Selçuk Kaplan, Sertaç Atalay

Abstract


Calpastatin (CAST) is an endogenous and specific inhibitor of calpains found in meat. Because of this feature, CAST is a major gene that directly changes the toughness of meat. The objective of this study to determine the CAST gene polymorphisms in Kivircik crossbred ewes. Therefore, CAST gene polymorphisms were investigated in 100 Kivircik crossbred ewes grown in Thrace region. The PCR-RFLP method was used to determine the genetic variations of the CAST gene. In the current study, the estimated frequencies of three genotypes including MM, MN and NN at CAST/MspI polymorphism were 0.82, 0.16 and 0.02 and they were 0.90 and 0.10 for M and N alleles, respectively. There was no deviation from Hardy-Weinberg equilibrium (P>0.05) relative to  CAST genotypes

Keywords


Kivircik Calpastatin Polymorphism RFLP

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References


Allais S, Journaux L, Levéziel H, Payet-Duprat N, Raynaud P, Hocquette JF, Lepetit J, Rousset S., Denoyelle C. & Bernard-Capel C. (2011) Effects of polymorphisms in the calpastatin and µ-calpain genes on meat tenderness in 3 French beef breeds. Journal of animal science 89, 1-11.

Asadi N, Nanekarani S. & Kherderzadeh S. (2014) Genotypic frequency of calpastatin gene in lori sheep by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. African Journal of Biotechnology 13.

Avanus K. (2015) Genetic Variability of CAST Gene in Native Sheep Breeds of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, 789-94.

Balcıoğlu MS, Karslı T, Şahin E, Ulutaş Z. & Aksoy Y. (2014) Türkiye’de Yetiştirilen Bazı Yerli Koyun Irklarında Kalpastatin (CAST) Geni Polimorfizminin PCR-RFLP Yöntemiyle Belirlenmesi. Tarım Bilimleri Dergisi 20, 427-33.

Byun S, Zhou H, Forrest R, Frampton C. & Hickford J. (2008) Association of the ovine calpastatin gene with birth weight and growth rate to weaning. Animal genetics 39, 572.

Dinh NTT. (2006) Meat quality: understanding of meat tenderness and influence of fat content on meat flavor. Science & Technology Development 9, 12.

Ferguson D. & Warner R. (2008) Have we underestimated the impact of pre-slaughter stress on meat quality in ruminants? Meat science 80, 12-9.

Gao Y, Zhang R, Hu X. & Li N. (2007) Application of genomic technologies to the improvement of meat quality of farm animals. Meat science 77, 36-45.

Georgieva S, Hristova D, Dimitrova I, Stancheva N. & Bozhilova-Sakova M. (2015) Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in Synthetic Population Bulgarian Milk sheep using PCRRFLP. Journal of BioScience & Biotechnology 4.

Glitsch K. (2000) Consumer perceptions of fresh meat quality: cross-national comparison. British Food Journal 102, 177-94.

Juszczuk-Kubiak E, Flisikowski K, Wicińska K, Połoszynowicz J. & Rosochacki S. (2009) Identification of the new polymorphisms in the promoter region of the CAST gene in cattle. Meat science 82, 278-83.

Kawasaki H. & Kawashima S. (1996) Regulation of the calpain-calpastatin system by membranes (review). Molecular membrane biology 13, 217-24.

Khederzadeh S. (2011) Polymorphism of calpastatin gene in crossbreed Dalagh sheep using PCR-RFLP. African Journal of Biotechnology 10, 10839-41.

Koohmaraie M. (1996) Biochemical factors regulating the toughening and tenderization processes of meat. Meat science 43, 193-201.

Koohmaraie M. & Geesink G. (2006) Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. Meat science 74, 34-43.

Li Y, Jin H, Yan C, Seo K, Zhang L, Ren C. & Jin X. (2013) Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China. Molecular biology reports 40, 1875-81.

Mohammadi M, Nasiri MB, Alami-Saeid K, Fayazi J, Mamoee M. & Sadr A. (2008) Polymorphism of calpastatin gene in Arabic sheep using PCR-RFLP. African Journal of Biotechnology 7.

Morris C, Cullen N, Hickey S, Dobbie P, Veenvliet B, Manley T, Pitchford W, Kruk Z, Bottema C. & Wilson T. (2006) Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey× Limousin, Angus and Hereford‐cross cattle. Animal genetics 37, 411-4.

Nassiry MR, Tahmoorespour M., Javadmanesh A., Soltani M. & Foroutani Far S. (2006) Calpastatin polymorphism and its association with daily gain in Kurdi sheep. Iranian Journal of Biotechnology 4, 188-92.

Nikmard M, Molaee V, Eskandarinasab MP, Dinparast Djadid N. & Vajhi AR. (2012) Calpastatin polymorphism in Afshari sheep and its possible correlation with growth and carcass traits. Journal of Applied Animal Research 40, 346-50.

Nuernberg K, Dannenberger D, Nuernberg G, Ender K, Voigt J, Scollan N, Wood J, Nute G. & Richardson R. (2005) Effect of a grass-based and a concentrate feeding system on meat quality characteristics and fatty acid composition of longissimus muscle in different cattle breeds. Livestock Production Science 94, 137-47.

Palmer B, Roberts N, Hickford J. & Bickerstaffe R. (1998) Rapid communication: PCR-RFLP for MspI and NcoI in the ovine calpastatin gene. J. Anim. Sci 76, 1499-500.

Pinto L, Ferraz J, Meirelles F, Eler J, Rezende F, Carvalho M, Almeida H. & Silva R. (2010) Association of SNPs on CAPN1 and CAST genes with tenderness in Nellore cattle. Genetics and Molecular Research 9, 1431-42.

Riaz MN, Ghaffar A. & Khan MFU. (2012) Calpastatin (CAST) gene polymorphism and its association with average daily weight gain in Balkhi and Kajli sheep and Beetal goat breeds. Pakistan Journal of Zoology 44.

Rosenvold K. & Andersen HJ. (2003) Factors of significance for pork quality—a review. Meat science 64, 219-37.

Saleha Y. (2015) Alakilli Analysis of Polymorphism of Caplstatin and Callipyge Genes in Saudi Sheep Breeds Using PCR-RFLP Technique Int. J. Pharm. Sci. Rev. Res 30, 340-4.

Santos-Silva J, Mendes I. & Bessa R. (2002) The effect of genotype, feeding system and slaughter weight on the quality of light lambs: 1. Growth, carcass composition and meat quality. Livestock Production Science 76, 17-25.

Schenkel F, Miller S, Jiang Z, Mandell I, Ye X, Li H. & Wilton J. (2006) Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. Journal of animal science 84, 291-9.

Sunilkumar M, Nagaraja C, Jayashankar M, Fairoze N. & Veeregowda B. (2014) Molecular studies on meat quality gene in Bandur sheep. Journal of Cell and Tissue Research 14, 4049.

Sutikno S, Yamin M. & Sumantri C. (2011) Association of polymorphisms Calpastatin gene with body weight of local sheep in Jonggol, Indonesia. Media Peternakan 34.

Szkudlarek-Kowalczyk M, Wiśniewska E. & Mroczkowski S. (2011) Polymorphisms of calpastatin gene in sheep. Journal of Central European Agriculture 12, 0-.

Tohidi R. (2013) Molecular Analysis of Ovine Calpastatin Gene in Six Iranian Sheep Breeds Using PCR-RFLP. Journal of Animal Production Advances 3, 271-7.

Yeh FC, Yang RC, Boyle TB, Ye Z. & Mao J.X. (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular biology and biotechnology centre, University of Alberta, Canada 10.

Yilmaz O, Sezenler T, Ata N, Yaman Y, Cemal I. & Karaca O. (2014) Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds. Turkish Journal of Veterinary and Animal Sciences 38, 354-7.

Zhou H. & Hickford J. (2008) Allelic polymorphism of the caprine calpastatin (CAST) gene identified by PCR–SSCP. Meat science 79, 403-5.




DOI: http://dx.doi.org/10.15316/SJAFS.2017.47

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