Determination of Inhibition Effect of Propolis Extract on Watermelon mosaic virus in Edible Seed Squash

Serkan Yeşil

Abstract


Edible seed squash (Cucurbita pepo L, ESS), which is grown for its seeds to consume as a snack, has a very important place in vegetable production in Türkiye. Viruses are one of the most problems in the cultivation of ESS in the country. One of these diseases is Watermelon mosaic virus (WMV, Potyvirus, Potyviridae). Also, it’s very common and devastating on other cucurbits worldwide. Propolis, which is composed mainly by the plant resins and exudates that honey bees gather, showed antiviral activity against plant and human viruses. In this study, the potential of propolis to control WMV was investigated by taking advantage of these antimicrobial properties of propolis. For this purpose, propolis extracted with 95% ethanol and diluted in distilled water to obtain different concentrations as 2, 4, 6, 8 and 10% were used. Effects of propolis against WMV were determined by in vitro and in vivo studies. Except of 2, and 4%, all concentrations caused to symptoms reductions of the infection, in all studies. In the result of in vitro studies, the ratios of healthy plants were calculated as 10, 20, 40% and 20, 30, 50% after one and two hours, respectively. In vivo, by spraying concentrations of 6, 8 and 10% before inoculation obtained healthy plants as 20, 40 and 60% after one-hour period, and as 20, 30 and 50% after two-hour period, while the extracts sprayed after inoculation, ratios of healthy plants as 10, 20 and 30% after one-hour period, and as 10, 20 and 20% after two-hour period. According to the results of the study, it was determined that applications of different propolis concentrations had the potential to reduce WMV infection, and these results should be supported by field trials.

Keywords


Edible seed squash; Infection; Propolis; Symptom; WMV

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References


Abd El Hady, FK, Hegazi AG (2002). Egyptian propo-lis: 2. Chemical composition, antiviral and antimic-robial activities of East Nile Delta propolis. Z. Na-turforsch C J Biosci. 57 (3-4), 386-394 10.1515/znc-2002-3-431 PubMed.

Anonymous (2022). Zirai Mücadele Teknik Talimatla-rı, (Erişim tarihi: 07/11/2022) https://www.tarimorman.gov.tr/TAGEM/Belgeler/Tek-nik%20tal%C4%B1matlar%202008/C%C4%B0LT%203.pdf

Bankova V, Marcucci MC, Simova S, Nikolova N, Kujumgiev A, Popov S (1996). Antibacterial diter-penic acids from Brazilian propolis. Z. Naturforsch., C, J. Biosci. 51 (5-6), 277-280. Doi: 10.1515/znc-1996-5-602

Bufalo MC, Figueiredo AS, de Sousa JPB, Candeias JMG, Bastos JK, Sforcin JM (2009). Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR. J. Appl. Microbiol. 107 (5), 1669–1680. PubMed http://dx.doi.org/10.1111/j.1365-2672.2009.04354.x

Coelho GR, de Senna Villar K, Figueiredo CA, Badari JC, Mendonça RMZ, Oliveira MI, Curti SP, Silva PES, Do Nascimento RM, Mendonça RZ (2014). Antiviral effects of Scaptotrigona postica propolis and their fractions. BioMed Central BMC Procee-dings 8 (No. Suppl 4), 63.

Doğan N, Hayoğlu İ (2012). Propolis and areas of usage. Journal of the Faculty of Agriculture of Harran University (Turkey) 16 (3), 39-48.

Fahmy FG, Omar MO (1989). Effect of propolis extract on certain potato viruses. Paper presented at: 4thInternational Conference on Apiculture in Tropical Climates (Cairo, Egypt). Gaba, V., Zelcer, A., and Gal-On, A. (2004). Cucurbit biotechnology-the importance of virus resistance. In Vitro Cell. Dev. Biol. Plant 40 (4), 346-358 http://dx.doi.org/10.1079/IVP2004554.

Gaba V, Zelcer A, Gal-On A (2004). Cucurbit biotechnology-the importance of virus resistance. In Vitro Cell. Dev. Biol. Plant 40 (4), 346-358 http://dx.doi.org/10.1079/IVP2004554.

Ghisalberti EL (1979). Propolis: a review. Bee World 60 (2), 59-84 http://dx.doi.org/10.1080/0005772X.1979. 11097738.

Kaya A, Erkan S (2011). İzmir, Aydın, Manisa ve Balıkesir İllerinde Üretilen Kabakgillerdeki Viral Etmenlerin Tanılanması ve Yaygınlıklarının Belirlenmesi (Identification of Viruses on Cucurbit Plants and Determination Their Prevalences in İzmir, Aydın, Manisa and Balıkesir Provinces). Bitki Koruma Bülteni 51 (4): 387-405.

Korkmaz F, Topkaya Ş, Yanar Y (2018). Tokat Kaba-kgil Üretim Alanlarında Enfeksiyon Oluşturan Virüslerin Belirlenmesi (Determination of Viruses in Cucurbit Production Areas in Tokat Province). Ga-ziosmanpaşa Bilimsel Araştırma Dergisi 7 (2): 46-56. https://dergipark.org.tr/tr/pub/gbad/issue/35699/368329

Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S (1999). Antibacterial, antifun-gal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol 64 (3), 235-240. PubMed http://dx.doi.org/10.1016/S0378-8741(98)00131-7

Marcucci MC (1995). Propolis: chemical composition, biological properties and therapeutic activity. Api-dologie (Celle) 26 (2), 83–99 http://dx.doi.org/10.1051/apido:19950202.

Mohamed EF, Owayss AA (2005). An inhibitory acti-vity of propolis extract against Broad bean mottle bromovirus (BBMV). Int. J. Virol. 1 (1), 313–321 10.3923/ijv.2005.31.31.

Sharifi M, Massumi H, Heydarnejad J, Pour AH, Shaabanian M, Rahimian H (2008), Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran, Virus Genes, 37 (3), 304-313.

Şevik MA, Balkaya A (2015). Samsun, Sinop ve Bolu İllerindeki Bal Kabağı (Cucurbita moschata Duch) Popülasyonlarına Ait Tohum Örneklerinde Virüslerin Tanılanması ve Bulunma Durumlarının Belirlenmesi (The Detection and Occurrence of Vi-ruses on the Seed Lots of Pumpkin (Cucurbita moschata Duch) Populations in Samsun, Sinop, and Bolu Provinces). Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi 32 (3): 70-77.

Takemura T, Urushisaki T, Fukuoka M, Hosokawa-Muto J, Hata T, Okuda Y, Hor, S, Tazawa S, Araki Y, Kuwata K (2012). 3, 4-dicaffeoylquinic acid, a major constituent of Brazilian propolis, increases TRAIL expression and extends the lifetimes of mi-ce infected with the influenza a virus. Evid Based Complement Alternat Med, 946867. PubMed http://dx.doi.org/10.1155/2012/946867

Topkaya S, Ertunc F (2012). Current status of virus infections in cucurbit plantations in Ankara and Antalya provinces. Proceedings of the Xth EU-CARPIA Meeting on Genetics and Breeding of Cu-curbitaceae, 759-762.

TÜİK (2022). Türkiye istatistik kurumu bitkisel üretim istatistikleri sorgulama sayfası. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim tarihi: 07.11.2022).

Vučurović A, Stanković I, Nikolić D, Milojević K, Bula-jić A, Krstić B (2017). Effect of propolis extract on Zucchini yellow mosaic virus inhibition in oilseed pumpkin. Acta Hortic. 1164, 431-438. DOI: 10.17660/ActaHortic.2017.1164.56 https://doi.org/10.17660/ActaHortic.2017.1164.56

Vural H, Eşiyok D, Duman İ (2000). Kültür Sebzeleri (Sebze Yetiştirme) EÜ Ziraat Fakültesi Bahçe Bitkileri Bölümü, EÜ Basımevi, s, 440.

Yeşil S (2019a). Some Virus Diseases of Edible Seed Squash (Cucurbita pepo L.) in Aksaray Province, Turkey. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 29: 63-71. https://doi.org/10.29133/yyutbd.476052

Yeşil S (2019b). Detection of Viruses on Edible Seed Squash (Cucurbita pepo L.) in Yozgat Province, Turkey. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 9 (3): 1202-1219. https://doi.org/10.21597/jist.509235

Yeşil S (2020). Detection of Some Virus Diseases of Edible Seed Squash (Cucurbita pepo L.) in Nevşehir Province, Turkey. Selcuk J Agr Food Sci 34 (1): 49-56. http://dx.doi.org/10.15316/SJAFS.2020.194

Yeşil S (2021a). Detection and molecular characterization of viruses infecting edible seed squash in Turkey. J Plant Dis Prot 128, 1341-1355. https://doi.org/10.1007/s41348-021-00477-4

Yeşil S (2021b). Determination of Inhibition Effect of Propolis Extract on Cucumber mosaic virus in Zucchini Squash. In: The Proceedings Book of 2nd International Congress of the Turkish Journal of Agriculture-Food Science and Technology. 373-378.

Yılmaz MA, Lecoq H, Abak K, Baloglu S, Sarı N (1992). Türkiye’de kabakgil sebze türlerinde zarar yapan virüsler (Pathogen Viruses on Cucurbit Vegetables in Turkey). Türkiye I. Ulusal Bahçe Bitkileri Kongresi, İzmir, Bildiriler kitabı, 439-442.

Yılmaz E (2014). Edirne İli Sebze Üretim Alanlarındaki Virüs Hastalıklarının Saptanması Üzerine Araştırmalar (Researches on Determination of Virus Diseases on Vegetable Production Areas in Edirne Province). Namık Kemal Üniversitesi. Yüksek Lisans Tezi. Fen Bilimleri Enstitüsü. Bitki Koruma Anabilim Dalı. 45s.




DOI: https://doi.org/10.15316/SJAFS.2022.069

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