Doublehaploidization Efficiency of Selected Pepper Genotypes Via in Vitro Anther Culture

Münevver İlhan, Ertan Sait Kurtar

Abstract


This study was carried out to determine the effects of genotype and media on haploid plant formation of 12 pepper genotypes (Capsicum annuum L.) via in vitro anther culture. The buds were cultured in 2 different media (O1 and O2) and, the results revealed that the media had effects on the development of anthers, embryo and plantlet initiation. When the anther developmental status is examined, the lowest value was obtained from the SU-31 genotype with 2.47%, while cv. Flinta F1 produced the highest rate with 12.79%. The highest growth rates were obtained from the O2 medium, and the cv. Dolphin variety produced a remarkable result with 25.0% in this medium. The cultivar Dolphin was the favorable genotypes with 8 embryos and 5 plants, while no embryo and plant were obtained from cv. Flinta F1 and SU-34. A total of 2868 anthers were cultured, 195 anthers were enlarged and the growth rate was 6.8%. Finally, 37 embryos and 18 plants were obtained and the frequencies were 1.29% and 0.63%, respectively. As a result of stomatal observations, 13 plants were detected haploid and the others were double haploid.

Keywords


Anther culture; Pepper; Doublehaploidization

Full Text:

PDF

References


Al Remi F (2013). Biberde (Capsicum annuum L.) anter kültüründe genotip ve besin ortamının etkisi. Çuku-rova Üniv. Fen Bilimleri Enstitüsü Yüksek Lisans te-zi, 55 s.

Al Remi F, Taşkın H, Sönmez K, Büyükalaca S, El-lialtıoğlu Ş (2014). Effect of genotype and nutrient medium on anther culture of pepper (Capsicum an-nuum L). Turk. J. Agric. Nat. Sci. 1, 108-116.

Arı E, Bedir H, Yildirim S, Yildirim T (2016). Androgenic responses of 64 ornamental pepper (Capsicum an-nuum L.) genotypes to shed-microspore culture in the autumn season. Turkish

Journal of Biology 40 (3): 706-717.

Atasoy D (2020). Bazı Biber (Capsicum annuum L.) Islah Genotiplerinin Anter Kültürü Performansla rının Belirlenmesi. Yüksek Lisans Tezi, Çukuro-va Üniversitesi, Adana.

Barroso P, Rêgo M, Rêgo E, Soares W (2015). Embryo-genesis in the anthers of different ornamental pep-per (Capsicum annuum L.) genotypes. Genetics and Molecular Res. 14 (4): 13349-13363.

Buyukalaca S, Kilic N, Comlekcioglu N, Abak K.,Ekbic E (2004). Effects of silver nitrate and donor plant growing conditions on production of pepper (Capsi-cum annuum L.) haploid embryos via anther culture. European J. of Hort. Sci. 69:206-209.

Çiner DÖ, Tıpırdamaz R (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish J. of Bot. 26 (3): 131-139.

Çömlekcioglu N, Buyukalaca, S, Abak K (2001). Effect of silver nitrate on haploid embryo induction by an-ther culture in pepper (Capsicum annuum L.). XIth EUCARPIA meeting on geneticsand breeding of capsicum and eggplant, Antalya, Turkey, 133-136.

Çömlekçioğlu N, Büyükalaca S, Abak K (1999).Şanlıurfa ve Kahramanmaraş biber popu-lasyonlarında anter kültürü yöntemiyle haploid bitki elde etme olanakları. Türkiye III. UlusalBahçe Bit. Kong. Ankara, 897-900.

Dumas de Vaulx RD (1989). Haploidy and pepper-breeding: a review. Capsicum News. (8-9): 13-17.

Dumas de Vaulx R, Chambonnet D, Pochard E (1981). In vitro anther culture in red pepper (Capsicum an-nuum L.): improvement of the rateof plant produc-tion in different genotypes by treatments at 35 oC. Agronomie, 1: 859-864.

Durna P (2016). Bazı Biber Genotiplerinde In Vitro Androgenesis Uygulamalarının Dihaploid Hat Geliştirmeye Etkisi ve Dihaploid Hatların Morfolojik Karakterizasyonu. Doktora Tezi, Tokat Gazi-osmanpaşa Üniversitesi, TOKAT.

Ellialtıoğlu Ş., Kaplan, F. ve Abak, K., 2001. The effect of carrot extract and activated charcoal on the an-drogenesis of pepper. Proceedings of XIth EU-CARPIA Meeting on Genetics and Breeding of Cap-sicum & Eggplant, 9-13.

FAO (2019). http://www.fao.org/faostat/ ErişimTarihi 15.02.2021.

George L, Narayanaswamy S (1973). Haploid Capsi-cum through experimental androgenesis. Proto-plasma, 78 (4): 467-470.

Irikova T, Grozeva S, Rodeva V (2011b). Anther cul-ture in pepper (Capsicum annuum L.) in vitro. Acta Phys. Plantarum, 33 (5): 1559-1570.

Irikova T, Grozeva S, Popov P, Rodeva V, Todorovska E (2011a). In Vitro Response of Pepper Anther Cul-ture (Capsicum annuum L.) Depend-ing on Geno-type, Nutrient Medium and Duration of Cultivation. Biotechnology & Biotec. Equipment, 25 (4): 2604-2609.

Karakullukçu Ş, Abak K (1992). Bazı patlıcan çeşit-lerinin anter kültürüne tepkileri. Ankara Üniversitesi Ziraat Fakültesi Yıllığı, 42: 7-12.

Keleş D, Pınar H, Ata A, Taşkın H, Yıldız S, Büyükalaca S (2015). Effect of pepper types on ob-taining spontaneous doubled haploid plants via an-ther culture. HortScience, 50 (11): 1671-1676.

Kim M, Jang IC, Kim JA, Park EJ, Yoo, M, Lee Y (2008). Embryogenesis and plant regeneration ofhot pepper (Capsicum annuum L.) through isolated mi-crospore culture. Plant Cell Reports, 27(3): 425-434.

Koleva-Gudeva LR, Spasenoski M, Trajkova F (2007). Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media. Sci. Hort. 111 (2): 114-19.

Kristiansen K, Andersen SB (1993). Effects of donor plant temperature, photoperiod, and age on anther culture response of Capsicum annuum L..Euphytica 67 (1): 105-109.

Lantos C, Juhász AG, Somogyi G, Ötvös K, Vági P, Mihály R, Kristóf Z, Somogyi N, Pauk J (2009). Im-provement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tis-sues of pepper or wheat. Plant Cell,Tiss. and Organ Cult. 97 (3): 285-293.

Nowaczyk P, Olszewska D, Kisiała A (2009). Individual reaction of Capsicum F2 hybrid genotypes in anther cultures. Euphytica, 168 (2):225-233.

Özsoy B (2019), Genotip Besin Ortamı ve Stres Uygu-lamalarının Biberde Androgenesis Üzerine Etkileri. Yüksek Lisans Tezi, Tokat Gaziosmanpaşa Üniver-sitesi, TOKAT.

Parra-Vega V, Renau-Morata B, Sifres A, Seguí-Simarro JM (2013). Stress treatments and in vitro culture conditions influence microspore embryo genesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.). Plant Cell, Tiss. and Organ Cult. 112 (3): 353-360.

Qin X, Rotino G (1993). Anther culture of several sweet and hot pepper genotypes. International Symp. on Cultivar Improvement of Hort. Crops.Part 1: Vege-table Crops 402: 313-316.

Rodeva V, Cholakov T (2006). Influence of some cli-matic factors in the period of donor plants growing on responsiveness of pepper anthers to embryogen-esis. The international conference haploids in high-er plants III, Vienna, Austria, 12-15.

Shtereva L, Zagorska N, Dimitrov B, Kruleva M, Oanh H (1998). Induced androgenesis in tomato (Lyco-persicon esculentum Mill). II. Factors affecting in-duction of androgenesis. Plant Cell Reports, 18 (3): 312-317.

Taskin H, Büyükalaca S, Keles D, Ekbiç E (2011). In-duction of microspore-derived embryos by anther culture in selected pepper genotypes. Afr. J. of Bio-tec., 10 (75): 17116-17121.

TÜİK (2020). http://www.tuik.gov.tr Erişim Tarihi: 15.02.2019.

Wang YY, Sun CS, Wang CC (1973). The induction of the pollen plantlets of triticale and Capsicum annu-um from anther culture. Scientia Sinica 16 (1): 147-151.




DOI: https://doi.org/10.15316/SJAFS.2022.033

Refbacks

  • There are currently no refbacks.


Creative Commons Lisansı
Bu eser Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.